Today, I found out I had an error in my transformation strategy to construct the red sensor-inverter-T7 wire-RFP cell. The error was twofold:

  1. Transforming the wrong plasmid into the correct strain.
  2. Using the wrong antibiotic selection.

Recently, I’ve been making too many novice experimental mistakes. Somewhere around 4 mistakes in a week, including poor planning, is my threshold. I think it means it’s time for me to take a step back from doing experiments, take some time to write up where I am w.r.t. each project I’ve been working on, and get my thoughts back in order.