Research Progress

Sometimes, research seems to go just so frustratingly slowly. There’s a gazillion and one things that have to get done right before I can proceed.

For example, setting up controls. I’ve been attempting a bacteria-to-yeast light communication experiment, and after a few tries at the assay set up by my previous mentor Robin, I was unable to replicate her results. And hence I have embarked on a month-long journey to troubleshoot the source of the problem. Is it the cells? Is it the reagents? Is there something about the way I’m handling the experiment?

It’s been mentally challenging to ground my own expectations. I thought I could set the goal of wrapping up the project within a month and a half – end of December and whole of January. But I ended up using December to set up the equipment and reagents needed to conduct the experiment (which involved a bit of waiting time, as I had to order things during the holiday season), and used half of January so far to troubleshoot why I haven’t been able to get yeast and bacteria to communicate.

That said, I’ve also started to think about ways to cheaply (time-wise) prototype bacterial color photography. I’m in the fortunate position of seeing interest from iGEM teams in accomplishing this goal, who are also producing parts that can be used. For example, simple protein reporters can be used to prototype the color output, before I hook up the actual pigment producing genes. I also have been trying to hook up Karsten’s T7 wires, based on advice from Jeff Tabor, but that did involve a short detour as I found my antibiotic plates weren’t good (the ampicillin probably went bad).

Also, I’ve received great advice from Mike, one of the post-docs in our lab, who gave me a few good ideas to think about related to gene expression control and genome-scale engineering.

Okay, time to continue working after a little hiatus. Onward!

Minimalism

Ever since there was a carpet refresh in our Tang Hall rooms, and we had to do a mid-stay mini-move (i.e. packing out all our things and moving it outside into the living room), I decided I’d try out Leo Babauta’s minimalistic style of living, keeping as few possessions as possible.

In order to reflect this, I have decided to keep my refreshed website as minimal as possible, especially in terms of page structures. As much as I can, I will keep things as blog posts, with little put into pages. And any content that can be not duplicated, such as my CV (which is already on LinkedIn), I will not duplicate.

Let’s see how this experiment goes…

Blog Restored!

I recently got hit by a malware infection (SQL injection, for the techie types out there, and I have no clue how it happened), and finally I have restored my blog. Apologies to everybody who have been following it all this while.

With that said, this is a prime opportunity to rebuild my website. Nothing from the past is lost (the text is still in a database file I’ve downloaded, and the old site’s files are backed up on my HDD and Time Capsule), and over time, I will curate and re-upload old posts back online.

In the meantime, to all my readers out there, enjoy the fresh start to 2012!

圣善夜,圣诞夜

今年的圣诞前夕,过得很特别。今年是我第一次穿着诗班的诗袍,在教会的会众前,以及在神的殿中献诗;诗班负责人皓芳姊妹所选的诗歌深具意义,并且教导有才,使我们从不会到会,我们虽技巧有缺却能不断提升,感谢神我们有她带领我们的诗班。

今年的圣诞前夕也是第一次独唱献诗,能在这天献诗,真是莫大的荣幸啊!献的是“圣善夜”(O Holy Night)。之前与钢琴伴奏陈育琪姊妹联系了不少次,感谢主在24日当天顺顺利利地献上诗歌,从中也体会到,若是为主的缘故,并且是以祂喜悦的方式来做,祂必定会赐力量,智慧,与能力来管理自己,克服困难,并且安排美好的结果。

以后再贴录分享分享!

A Semester Gone!

Wow!

What else can I say, but wow?

It’s been a whirlwind ride here at MIT during my first semester. Looking back, the last entry I wrote was at the beginning of semester, and only now, at the end of the semester, do I have the chance to write up something.

Back at the start of the semester, when things were a little lighter, I took stock of a few things I wanted to do in school throughout the first semester. Here they are:

  • MIT100K
  • Thesis
  • Korean Class
  • MIT iGEM
  • Sports

This, on top of regular school work of course.

Granted, school work did eat up a lot of time. But things have become clearer in the past semester as to how things will pan out for the coming semester.

MIT100K

I’ve moved from web team member to web team lead + marketing member. It’s been quite an interesting journey along the way. I’ve had the chance to converse with many Sloanies, and they bring a very different type of energy to the table. I really enjoy working with the 100K crowd, and I’m looking forward to continued involvement until the Business Plan Competition.

More importantly, I’ve learned quite a lot about business from an outsider’s lens, and I realize that a lot of it is commonsensical, except that in the excitement of doing a startup, perhaps one may lose sight of that common sense. Of course, that’s the theorist inside me speaking; if I were to one day get involved in a startup or industrial management work instead, perhaps my perspective might change.

Thesis…

…is more or less defined! And I have made more inroads into advancing the project further. Bacterial color photography. This IAP I will be beginning the first cloning steps to assemble the DNA, and with the 2nd semester around, I think I will be in the lab more often. This will mean balancing my studies better; in particular, making sure I’m reviewing the material right after each class rather than darting off.

Korean Class

1 hr 30 min every week, and then a drama episode or two when I have time, and I think I have made much progress in listening and understanding Korean. But my vocabulary is much too weak, and my confidence much too low, to be able to string together a sentence. Hence, I’m looking forward to moving onto the Intermediate level classes. Hopefully I’ll be able to make some progress here.

MIT iGEM

I’ll take Chris’ suggestion and keep my involvement low until I pass quals. This year might not be the year for me to step up just yet.

Sports

In this area, I haven’t done so well. During the semester, there were times when I went swimming, biking, and playing badminton, but there were stretches where I was too tired to go for it, or it was too hassle-some to go, or… okay, enough of the excuses. Basically, my primary care physician @ MIT Medical says I should have a 500 cal difference between intake and output, with intake < output, to lose weight properly, and I no longer have an excuse for being this much overweight. The coming semester is a time when I think I’ll raise my game more. I hope to go for more regular badminton games now, starting from tomorrow’s badminton game with Ian.

School has started

It’s been a while since I last had the chance to write up here. School has started, things are ramping up, and I’m finding quite a lot less time to do writing like this.

Since this is my first year back in school after a year and a half hiatus from being in a learning community, and because I’ve got at least 4 years here in total, I decided to spend the first year exploring around, to see what opportunities lie awaiting, and what opportunities I might be able to help create.

MIT $100K Competition

It’s an entrepreneurship competition. But for now, I’d like to get back into the teamwork groove by helping out with the organizational side of things, rather than do any actual entrepreneurship work. Things are looking positive here.

Thesis

Thesis project idea has been defined, I’m essentially taking on color bacterial photography. It’s a “handed-to-me” project, but I’m trying to anticipate ways I could work with others, as well as potential problems that I would need to solve that would help advance the field forward. We’ll see how that goes.

Korean Classes

Apparently there are Korean language classes offered for free here! No word on the location yet, so no confirmation that it’s actually on, but I’ll hope to hear back from the instructors/organizers on this.

MIT iGEM

iGEM remains in full force here, and after talking with a few guys in the Weiss lab, I think it’s something I’d definitely explore, after 1st year ends, of course.

Sports

Badminton, biking, basketball. It’s time to get back into shape. The past week I’ve played bball twice, and though it really showed how unfit I am right now, it was real fun to get back into running around, thinking strategically on the court, and shooting some hoops. Once my rackets come, I’m totally going to play some badminton as well.

Challenges

Looking forward, the coming year certainly looks like it’ll be exciting, including classes of course, which I guess I will have to try to make my way through. That is the one part of school work where I think I will most certainly be depending working through things with my fellow classmates.

24

And so another year in my life has officially passed by.

As planned, I crossed the threshold point with little fanfare. Last year, the count was 6, excluding well-wishing from my family and people who recently came to know. This year, using the same criteria, it was 1, and it wasn’t even posted on a public “Facebook wall”.

My goal has well and truly been accomplished. I never intended it to be an experiment when I started taking down 110726 from my profile pages, but by the time I hit university, I started thinking, how many people could remember it without having to refer to Facebook to find out? Also, how low could I have the number go?

I decided to carry out a long experiment in interactive social psychology (call that a term I conjured up in a whirlwind), risking, of course, some degree of alienation, scratching of heads and weird looks. In would insist that I did not do anything overtly special on this day, and that none of my friends do anything special or buy me anything. At most, I allowed myself a special food treat, like a mocha rather than coffee. I was my own treatment group. The only downside was I had no control group – unless you considered all of my normal friends who celebrated their birthdays normally.

A few years back, I wrote a mock-paper on the subject, and found that friends are incredibly forgetful, even with reminder tools.

Combine that with my insistence to keep today as normal and low-key as possible, means that for once, I had a less-than-normal day on the 24th anniversary of the day I was born.

Today looked like this: Go to work, encounter a really bad interpersonal problem at work, try to resolve problem at work, come home with a heavy heart, ask in prayer for peace and wisdom to deal with it, then find a future potential problem.

Breakfast: As usual.

Lunch: A sandwich I hadn’t eaten at the cafe in over 2 months now.

Dinner: A soup invention with noodles; leftover cheesecake from another friend’s birthday dinner for dessert.

What a day eh?

I have decided to stop this experiment. In some respects, risking the scratching heads and weird looks of misunderstanding was worth it. I confirmed my own hypotheses, and reached an unbelievably low number of ‘HBDs’ for the average city slicker. Concealing my own birth date also served to protect myself from the undesirable advances of others who might want to ‘persuade’ me to down ethanol on this day.

But I have found myself also becoming sensitive to the joy and happiness others have when they share their special days with other friends, and I don’t want to risk this sensitivity turning into cynicism and bitterness. That would be simply a big, big mistake.

I still won’t reveal on my public profile pages the day I was born or my age. But I will no longer insist that friends do nothing for me. From this point onwards, I will gladly accept if they decide to do anything edifying (ethanol pushers read: encourage intellectual, moral, or spiritual improvement, so I am saying NO to EtOH still), like having a chat about life over coffee, or learning from seniors how to deal with challenges ahead in school, or sharing about deeply meaningful and purposeful living.

(Side note: I find that the higher I probe in grad school, the fewer deeply purposeful people I can find… and instead, I see a lot more of self-servitude, survival-of-the-fittest scarcity mentalities, and cynicism, be it overt, subtle or subconscious in nature.)

Anyways, it is late now, and is already the next day. Time to move on, and time to sleep.

The Richness of Our Lives

What do you have that you can give away?

Is it money? Skills? Talent? Time? Knowledge?

More often than not, my epiphanies come from being in the washroom. This particular epiphany is no exception, coming while showering.

We all possess something that can be given away. More often than not, we have more than one of those four things that we can give away.

That is the richness of our life. When we give away one of these things, it shows that we are rich in those things.

If I cling to those things, to my money, to my time, to hide my talent or skills, it tells of an attitude of impoverishment.

Yet the whole irony behind giving these things away is that invariably, the better you steward them for the benefit of others, the more you will receive back. That is called an investment of riches.

On the flip side, the more you try to keep them to yourself, the more impoverished you will be. That is called indulgence towards impoverishment.

We’re all given things with which we can choose to invest in others, or indulge for ourselves.

What will you choose?

“The more you give, the more you will receive.” – Unknown
“Whoever has will be given more; whoever does not have, even what they have will be taken from them.” (Mark 4:25 NIV)

Packing

Yet again, it comes to the point that I must pack up an leave. My journey continues on. San Francisco was a stop en route to Boston, just as UCSF was a stop en route to MIT. (I’ve been in biotech cities, come to think of it.)

This time, packing is much easier. I have much less gunk than before. My life has been much more streamlined and digitized, save for a few nooks and crannies.

Throughout the process, I have realized one thing, confirmed in a blog post on the Zen habits blog: experiences > possessions. The way I see it, possessions are but a tool – a tool for bringing new experiences or making a continuing one enhanced further.

I hope that in the following years, I don’t fall into the possessions trap, but rather that I come to create shared experiences with my friends and, God willing, my mom and brother as well, for I think we’ve had one to few together.

I diverge. Packing, as a result of being streamlined, has been made much easier. Digitizing paperwork, much of which I hardly access anyways, lightens a ton of a load. Add in a few other hacks to make packing more compact, and that makes for a simpler move.

What have I left to pack? No, I shan’t post a laundry list here. Clothes, computer, and perhaps one or two kitchen implements I’ve inherited, and that concludes it.

And yet again, a post on some practical life matter becomes an excuse to write about philosophical thoughts instead.

Teaching Philosophy

Before you continue reading this page, it is my hope that you come to understand why I have written it in the first place. Yes, it is the “WHY”, to borrow a phrase from Simon Sinek, that informs my writing of this page. I formulated this “WHY” statement based on months and months of reflection on what it means to be a teacher, and after a farewell dinner the 2010 UBC iGEMers, everything finally came together. I hope you will come to enjoy this page.

“WHY” do I want to be a professor, a teacher of students, an inspirer of minds? That is because <strong>I believe that the power of inspiration and empowerment can be used to better the world</strong>. An student must be inspired by his/her teacher’s example. And then the student must be given the tools to empower them to do greater things. When a student is inspired towards a greater cause, and is empowered with the tools to make the cause a reality, that is when the magic begins.

Inspiration begins with the teacher’s infectious excitement and energy. Empowerment, then, starts with technical knowledge, but must expand to include building confidence (e.g. presenting the project, providing a leg-up in networking), providing tangible support (e.g. reference letters), and building emotional resilience (e.g. one-to-one chatting when rough times come).

When inspiration and empowerment are present, the student becomes less of a student and more of a colleague and a friend. That, then, is when the teacher has been successful. That is when other people may now benefit from the teacher-student relationship, even if they were not directly involved, when the student, now a colleague, lights up another candle of inspiration and passes it on.

That concludes my “WHY”. The “HOW”, which is informed a lot by the empowerment part of my “WHY”, is what I have written below.

My undergraduate education has shaped the way that I view teaching. Thus, I have formulated a view of teaching, where I believe in:

(1) Problem-Based Learning

The biggest skills a student should have is the ability to go through the process of coming up with a solution to a problem. For hypothetical scenarios, I believe that the solution is not as important as the process of coming up with one. However, when translated into real-world scenarios, students should be able to come up with solutions that consider constraints imposed upon them by reality. It is not important, however, to come up with a the final solution on first pass of the problem. It is important, rather, to come up with a first iteration of a solution that can be practically refined further.

(2) Concepts over Details

I believe that students ought to place their learning emphasis on generalizable concepts and principles as opposed to minute details. I alluded to this in an article I wrote about the Microbiology and Immunology undergraduate curriculum. Equipped with generalizable concepts and principles, students can approach a multitude of problems and figure out a first approximation of a solution before further refining it.

(3) Intuiting Knowledge

By placing emphasis on concepts and not details, students can assimilate their knowledge into their intuition. Intuition is a very useful skill in figuring out the first iteration of a solution before further refining it.

(4) Learning How To Find and Organize Information

In an online, connected world, there is little need to memorize details, as the details can be found at the touch of a finger, or a click of a button. However, it is more important for a student to be able to organize information in a logical fashion, for the information out there is not organized. Organization also inadvertently aids retention.

Organization takes place on two levels: for personal understanding, and for communication to others. The organization of information should highlight the hierarchy of knowledge about that subject matter, while demonstrating the interactions between each component within the hierarchy of knowledge.

(5) Effectively Communicating Ideas

A student ought to be equipped with the capability to communicate what they know, and emphasis should be made on communication right from the start of an undergraduate education. A student should be equipped with the necessary vocabulary to deal with a wide ranging audience, from the layman to the specialist, and be able to appeal to all three modes of learning: visual, aural and kinesthetic.

(6) Providing Space for Challenge

A student should be given the space to challenge him or herself towards increased depth of thought, broader horizons of exposure, and greater heights or achievement. This would facilitate the development of each student according to his or her level.

As an example, I would offer a limited number of my students (~10 only, chosen at random if necessary) such an opportunity through the use of an oral examination as a substitute for a written final examination. The rubrics for such an oral examination would essentially be similar to the written examination; however, fulfilling the rubrics would only guarantee a portion of the marks.  The other proportion would be earned by my questioning above and beyond the basic rubrics. (It is worthy to note that the written examination would have a similar, essay-based component.) The further a student goes before being fully stumped, the higher his or her grade will be.